Fig. 3: Dual-specificity of anti-DNA scFvs for pyrP.
From: Molecular and structural basis of anti-DNA antibody specificity for pyrrolated proteins
a List of scFv-clones established in Fig. 1d. b Binding of scFv DO1–DO8 to antigens (BSA, pyrBSA, and DNA). Antigens (1 μg/well) immobilized on the ELISA plate were incubated with monoclonal scFv-His6 at concentrations of 1, 10, and 100 nM. Binding was detected using the HRP-conjugated anti-His6-tag antibody. Data are mean ± S.D. of triplicate samples (representative of three independent experiments). c Schematic representation of dual-specificity of anti-DNA scFvs for pyrP.
