Fig. 5: The VH domain primarily contributes to the recognition of DNA and pyrP.
From: Molecular and structural basis of anti-DNA antibody specificity for pyrrolated proteins
a Binding of VH-His6 to antigens (BSA, pyrBSA, and DNA). Antigens (1 μg/well) immobilized on an ELISA plate were incubated with VH-His6 (1, 10, and 100 nM). Data are mean ± S.D. of triplicate samples (representative of three independent experiments). b Competitive ELISA for binding of VH DO1 (left) or VH DO2 (right) to DNA. The binding of VH-His6 to DNA was evaluated in the presence of single-stranded DNA (ssDNA) or double-stranded DNA (dsDNA) (12.5, 25, 50, and 100 nM each). (dN)20 indicates 20 bases of ssDNA, and (dN:dN)20 indicates 20 base pairs of dsDNA. ds(T7 primer) is double-stranded DNA with sequence of 5’-TAATACGACTCACTATAGGG-3’. Data are mean ± S.D. of triplicate samples (representative of three independent experiments).
