Fig. 6: Significance of arginine residues in VH domain Abs for recognition of DNA and pyrP.
From: Molecular and structural basis of anti-DNA antibody specificity for pyrrolated proteins
a Effect of ionic strength on antigen recognition of VH DO1 (left panel) and VH DO2 (right). Antigens (1 μg/well of pyrBSA or DNA) immobilized on ELISA plate were incubated with 10 nM VH-His6 under the indicated concentrations of NaCl. b and c Binding of arginine-substituted mutants of VH DO1 (VH DO1-R97A) and VH DO2 (VH DO2-R56N, VH DO2-R96A, and VH DO2-R99A) to antigens (pyrBSA (left panels) and DNA (right panels)). Data are mean ± S.D. of triplicate samples (representative of three independent experiments).
