Fig. 3: The roles of m6A in regulating KCTD21-AS1 activity. | Communications Biology

Fig. 3: The roles of m6A in regulating KCTD21-AS1 activity.

From: m6A-methylated KCTD21-AS1 regulates macrophage phagocytosis through CD47 and cell autophagy through TIPR

Fig. 3

a meRIP analysis of m6A-modified KCTD21-AS1 in A549 cells. b, c METTL14 overexpression increased, but siRNA-METTL14 inhibited KCTD21-AS1 levels in A549 cells. d, e Effect of METTL14 overexpression and siRNA-METTL14 on KCTD21-AS1 levels in H1975 cells. f, g CCK8 assay showed METTL14 overexpression promoted, whereas siRNA-METTL14 inhibited A549 cell proliferation. h, i METTL14 overexpression promoted, whereas siRNA-METTL14 inhibited H1975 cell growth. j Blocking KCTD21-AS1 attenuated the roles of METTL14 in increasing KCTD21-AS1 expression. k Blocking KCTD21-AS1 accelerated the roles of siRNA-METTL14 in decreasing KCTD21-AS1 expression. Data was presented as meanā€‰Ā±ā€‰SD for triplicate experiments. *pā€‰<ā€‰0.05; **pā€‰<ā€‰0.01; Studentā€™s t-test or ANOVA.

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