Fig. 4: miR-519d-5p suppressed lung cancer cell proliferation.

a The binding sites between miRNA and KCTD21-AS1. b, c miR-519d-5p regulated luciferase expression in A549 and H1975 cells. d RNA immunoprecipitation assays. miR-519d-5p and KCTD21-AS1 were estimated by qRT-PCR. e, f KCTD21-AS1 overexpression or siRNA-KCTD21-AS1 regulated miR-519d-5p levels in A549 and H1975 cells, respectively. g, h miR-519d-5p overexpression inhibited A549 cell proliferation. i, j miR-519d-5p inhibitor promoted A549 cell proliferation. k, l miR-519d-5p decreased, whereas ASO-519d-5p increased A549 cell colony formation. m–o Tumor size, weight, and volume changes were measured in miR-519d-5p- or control-treated xenografts, n = 5. p miR-519d-5p levels in xenografts. Data are presented as the mean ± SD or median (interquartile range). *p < 0.05; **p < 0.01; Student’s t-test, ANOVA, or Mann–Whitney U test.