Fig. 6: KCTD21-AS1 and miR-519d-5p regulated TIPRL expression.

a The sites of TIPRL-3’-UTR or Mu-TIPRL-3’-UTR targeted by miR-519d-5p. b, c Luciferase levels were analyzed in both pc3.1-luci-TIPRL-3’-UTR and miR-519d-5p-treated H1975 or A549 cells, respectively. Data were expressed as mean ± SD for triplicate experiments. **p < 0.01, Student’s t-test. d immunoblotting detection from different membrane, and the sample loading amount according to the reference control, which was run on a different gel than the corresponding sample of interest. e, f Effect of TIPRL recovery on the role of miR-519d-5p in H1975 or A549 cell growth using CCK8 assay, respectively. Data were expressed as mean ± SD for triplicate experiments. *p < 0.05, **p < 0.01; ANOVA. g, h Effect of TIPRL-3′UTR recovery on miR-519d-5p-regulating H1975 or A549 cell metastasis, respectively. Data were expressed as mean ± SD for triplicate experiments. Bar = 100 μm. *p < 0.05, **p < 0.01; ANOVA. i immunoblotting detection from different membrane, and the sample loading amount according to the reference control, which was run on a different gel than the corresponding sample of interest.