Fig. 7: KCTD21-AS1 and miR-519d-5p regulated CD47 expression.

a CD47-3’-UTR or Mu-CD47-3’-UTR targeted by miR-519d-5p. b Luciferase levels were analyzed in both pc3.1-luci-CD47-3’-UTR and miR-519d-5p-treated H1975 cells. Data were expressed as mean ± SD for triplicate experiments. **p < 0.01, Student’s t-test. c immunoblotting detection from different membrane, and the sample loading amount according to the reference control, which was run on a different gel than the corresponding sample of interest d immunofluorescence analysis of CD47 levels in H1975 cells. Bar = 20 μm. e Effect of CD47-3′UTR recovery on the role of miR-519d-5p in H1975 cell growth using CCK8 assay. Data were expressed as mean ± SD for triplicate experiments. *p < 0.05; ANOVA. f lv-miR-519d-5p treatment enhanced the phagocytic function of macrophages. Data were expressed as mean ± SD for triplicate experiments. **p < 0.01, Student’s t-test. g KCTD21-AS1 overexpression reduced the phagocytic function of macrophages. h siRNA-KCTD21-AS1 promoted the phagocytic function. Data were expressed as mean ± SD for triplicate experiments. **p < 0.01, Student’s t-test. Data were expressed as mean ± SD for triplicate experiments. **p < 0.01, Student’s t-test.