Fig. 1: Generation of RNAi-sensitive CDC20 knockout cell lines by CRISPR/Cas9.

a Cdc20 in knockout cells from three sgRNAs was examined by western blot with an antibody targeting the Cdc20 C-terminal region. See Supplementary Fig. 5 for uncropped blots. b–g The time from NEBD (nuclear envelope breakdown) to anaphase for unperturbed mitosis or NEBD to mitotic exit after microtubule toxin treatment. Each circle represents the time of a single cell, and the red line indicates the median time. The number of cells analyzed per condition is indicated above (n = X). A representative experiment of two independent experiments is shown. Mann–Whitney U-test was applied. ns means not significant; ** means P < 0.01; **** means P < 0.0001. b The mitotic time of the knockout cells. c The mitotic time of the knockout cells after depleting the residual endogenous Cdc20 by RNAi. Red dots are the cells that died after the metaphase arrest. d The mitotic time of cells treated with nocodazole (30 ng/ml). e The mitotic time of cells treated with paclitaxel (0.1 µM). f The mitotic time of cells treated with reversin (0.5 µM). g The mitotic time of knockout cells transfected with YFP-Cdc20 expression plasmid and treated with nocodazole (30 ng/ml).