Fig. 2: Functional analysis of Cdc20 in the null background.
From: Functional analysis of Cdc20 reveals a critical role of CRY box in mitotic checkpoint signaling
a Schematic of the positions of the motifs or phosphorylated sites analyzed in this study. b Mutation details and known functions of the motifs or phosphorylated sites in this study. c–f The time from NEBD to anaphase (left) or from NEBD to mitotic exit in the presence of nocodazole (right, 30 ng/ml) of the knockout cells complemented with wild-type or mutant YFP-Cdc20 and depleted the residual endogenous Cdc20 by RNAi. Each circle represents the time of a single cell, and the red line indicates the median time. The red circle means the cell died after mitotic arrest. The number of cells analyzed per condition is indicated above (n = X). A representative experiment of two independent experiments is shown. Mann–Whitney U-test was applied. ns means not significant. ** means P < 0.01;*** means P < 0.001; **** means P < 0.0001.
