Fig. 3: Structure analysis of the CRY motif of Cdc20^MCC in the context of MCC-APC/C.

a Structure of the APC^MCC complex (PDB ID: 6TLJ, Alfieri et al., 2020), the APC/C is shown as surface, the MCC subunits and Cdc20^APC/C are shown as cartoon representation. b Close-up view on the Cdc20^MCC CRY box region (from a), interacting with Cdc20^APC/C and BubR1^KNOT domain (gray cartoon). The surface electrostatic potential is shown for the Cdc20^APC/C. c, d Two close-up views showing the composite interface made of Cdc20^APC/C and BubR1^KNOT domain, which recognizes the Cdc20^MCC CRY box. Cdc20^APC/C is shown as a cartoon representation and transparent surface view in (c), as well as Cdc20^APC/C and BubR1^KNOT domain in (d). Cdc20^MCC is shown as a cartoon representation in both (c) and (d). Relevant residues are depicted in (b–d).