Fig. 5: The C box and CRY box are exchangeable for their proper functioning.
From: Functional analysis of Cdc20 reveals a critical role of CRY box in mitotic checkpoint signaling
a Schematic showing the design of the C box and CRY box exchanging. b The time from NEBD to anaphase (unperturbed) or from NEBD to mitotic exit in the presence of nocodazole (30 ng/ml) of the knockout cells complemented with wild-type or engineered YFP-Cdc20 and depleted the residual endogenous Cdc20 by RNAi. Each circle represents the time of a single cell, and the red line indicates the median time. The number of cells analyzed per condition is indicated above (n = X). A representative experiment of two independent experiments is shown. Mann–Whitney U-test was applied. ns means not significant. c,d sequence alignments showing the conservation of Cdc20 CRY BOX (c) and BubR1 KNOT domain (d). e Cartoon highlighting the critical role of the CRY box in mitotic checkpoint signaling. Top left: kinetochore (yellow circle) not attached by spindle microtubule (green line) organized by centrosome (gray squares) activates the spindle assembly checkpoint; Bottom left: MCC complex is generated from the unattached kinetochore. The CRY box is required for the complex formation. Right: Stable interaction between MCC and APC/C requires multiple interactions mediated by the CRY box.
