Fig. 6: Re-secretion of exosomal Wnt5a, but not Wnt11, is increased specifically by sFRP2.
From: Soluble Frizzled-related proteins promote exosome-mediated Wnt re-secretion
Exosome-mediated re-secretion of Wnt5a and Wnt11 was examined according to the procedure shown in Supplementary Fig. 4a (Re-secretion assay #2) for (a), or Fig. 1a (Re-secretion assay #1) for (b). Western blot analysis of S10 (2nd), S100 (2nd), and P100 (2nd) for examining the specificity of sFRP to Wnt5a (a) and Wnt11 (b) proteins. S100 mixture from separately cultured CSs of non-tagged Wnt5a/L cells and sFRP2-, sFRP3-, expressing or control HEK293 were used to treat MDCK cells in (a). Exosome-depleted CS of Wnt11-mClover/HEK293 cells co-cultured with sFRP2-, sFRP3-expressing or control HEK293 cells were used to treat MDCK cells in (b).
