Fig. 1: Schematic illustration of human myocardial slice acquisition and long-term cultivation and synchronized force and calcium measurements in biomimetic culture chambers (BMCCs).

Left ventricular tissue slices were acquired and cultured in vitro for >3 weeks. BMCCs were transferred to a custom-made fluorescence microscope system for synchronized measurement of contractility and calcium signal under electrical stimulation. The heart illustration is taken from Servier Medical Art, provided by Servier and licensed under a Creative Commons Attribution 3.0 Unported License.