Fig. 1: MR1 ligands delivered to antigen presenting cells on soluble MR1 molecules are efficiently recognized by MR1T cell clones. | Communications Biology

Fig. 1: MR1 ligands delivered to antigen presenting cells on soluble MR1 molecules are efficiently recognized by MR1T cell clones.

From: Delivery of loaded MR1 monomer results in efficient ligand exchange to host MR1 and subsequent MR1T cell activation

Fig. 1

a MR1T cell clone (1e4 cells) IFN-γ responses to MR1/5-OP-RU or MR1/6-FP tetramer, either added to DCs (1e4 cells) (exSPOT) or bound to ELISPOT plate (tetraSPOT). b MR1T cell clone (1e4 cells) IFN-γ response to MR1/5-OP-RU is inhibited by anti-MR1 antibody blockade (aMR1, 2 μg/ml). c MR1T cell clone (1e4 cells) IFN-γ response to MR1/5-OP-RU tetramer, added to either DCs, THP-1 cells, BEAS-2B (B2B) cells or B-LCL (1e4 cells). EC50 calculated using non-linear regression and the difference in the EC50 was statistically significant between the curves (p < 0.0001) by the extra sum-of-squares F-test. Data are representative of n = 3 independent experiments each. Technical duplicates within the representative experiment are shown with a line connecting the mean. For EC50 values of replicate experiments see Supplementary Tables 1, 2.

Back to article page