Fig. 2: Ligand delivered in the context of MR1 monomer is presented more efficiently than free ligand. | Communications Biology

Fig. 2: Ligand delivered in the context of MR1 monomer is presented more efficiently than free ligand.

From: Delivery of loaded MR1 monomer results in efficient ligand exchange to host MR1 and subsequent MR1T cell activation

Fig. 2

a MR1T cell clone (1e4 cells) IFN-γ response to DCs incubated with MR1/5-OP-RU tetramer, MR1/5-OP-RU monomer, and soluble 5-OP-RU. EC50 calculated using non-linear regression. The difference in the EC50 was statistically significant between the MR1/5-OP-RU tetramer and 5-OP-RU and between MR1/5-OP-RU monomer and 5-OP-RU curves (p < 0.0001), but there was no significant difference in the EC50 of MR1/5-OP-RU tetramer and monomer by the extra sum-of-squares F-test (p = 0.7486). Data are representative of n = 3 independent experiments. Technical duplicates within the representative experiment are shown with the mean and a line depicting the non-linear regression curve. For EC50 values of replicate experiments see Supplementary Table 3. b BEAS-2B.MR1-GFP cells were incubated with the indicated concentrations of MR1/5-OP-RU monomer or free 5-OP-RU overnight and surface stained for flow cytometry with APC-conjugated TCR tetramer. Representative histograms are shown with pooled geometric mean fluorescence intensity (GeoMFI) from n = 4 experiments. The same unstained and untreated control are shown in both histogram plots for easy comparison. Error bars denote SD of the mean of pooled experiments which are shown individually in lighter colors.

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