Fig. 1: GTP bound KRAS is required for RBDCRD to localize to the supported lipid bilayer. | Communications Biology

Fig. 1: GTP bound KRAS is required for RBDCRD to localize to the supported lipid bilayer.

From: Membrane lipids drive formation of KRAS4b-RAF1 RBDCRD nanoclusters on the membrane

Fig. 1

a TIRF images (150 pixel X 150 pixel, where 1 pixel is 0.16 µm) of JF646 labeled HaloTag RAF1 RBDCRD deposited onto a supported lipid bilayer composed of eight lipids in the absence of KRAS (b) in the presence of KRAS GDP (c) in the presence of KRAS GppNHp (non-hydrolysable analog of GTP), abbreviated as GNP in the figure. The bottom panel is the pictorial representation of the proteins and lipid bilayer shown throughout the manuscript. The cyan-blue ribbon-like structure represents the headgroups of the lipid bilayer, the dark blue globular structure represents KRAS, the green represents RBD and the yellow represents CRD.

Back to article page