Fig. 8: Mechanistic insights into RBDCRD-membrane interactions.
From: Membrane lipids drive formation of KRAS4b-RAF1 RBDCRD nanoclusters on the membrane
a Dot plot representation of NMR-PRE ratios (I/I0) plotted as (1-(I/I0) to compare with contact probabilities obtained from the MuMMI AA simulations. b HSQC spectra showing reduction in intensities of CRD residues of RBDCRD bound to DMPC:DMPS bicelles (red) upon the addition of doxyl-5 (blue), doxyl-14 (green) and Gd3+ (magenta). c Contact probability distribution of CRD residues in KRAS-RBDCRD as a function of the distance between the COM of hydrophobic/cationic CRD loop residues and the COM of the bilayer along the bilayer normal (dz), obtained from MuMMI AA simulations. d CRD residues with PRE ratios of ≤50% are mapped onto a model of RBDCRD: doxyl-5 and doxyl-14 (red) and Gd3+ (magenta). e Mean square displacement plots of KRAS on 8-lipid bilayer when bound to wild type RBDCRD (solid) and mutated RBDCRD K148A/K157A/K179A (dotted). The corresponding raw data with associated errors are tabulated in Supplementary Table 8.
