Fig. 2: The ISKNV genome is highly methylated, and almost all CpG sites are highly methylated in all contexts. | Communications Biology

Fig. 2: The ISKNV genome is highly methylated, and almost all CpG sites are highly methylated in all contexts.

From: Hypermethylated genome of a fish vertebrate iridovirus ISKNV plays important roles in viral infection

Fig. 2

a The average percentage of methylated and unmethylated cytosines obtained from WGBS after extraction by Bismark. b Methylation levels of CpG, CHG, and CHH. c Methylation levels of cytosines distinguished by the following 2 bases in the 5’ region. d Methylation levels of cytosines distinguished by the following 1 base in the 5’ region and the following 1 base in the 3’ region. e Methylation levels of cytosines distinguished by the following 2 bases in the 3’ region. f–h Distribution of methylation levels of CpG, CHG, and CHH classified by different contexts. i The overall distribution of CpG, CHG, and CHH methylation levels in the ISKNV genome. j The overall distribution of CpG, CHG, and CHH methylation levels in the R and L strands. k The overall distribution of CpG, CHG, and CHH methylation levels in ORF and NCR. l The overall distribution of CpG, CHG, and CHH methylation levels in the CGI and non-CGI. Data are shown as the mean ± SD (n = 3) in (b–h). Solid lines represent the mean and the dashed lines represent the upper and lower quartiles in (i–l). Statistical significance is indicated by asterisks, with *p-value < 0.05 and **p-value < 0.01.

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