Fig. 3: Molecular experiments demonstrated that the ISKNV genome is highly methylated. | Communications Biology

Fig. 3: Molecular experiments demonstrated that the ISKNV genome is highly methylated.

From: Hypermethylated genome of a fish vertebrate iridovirus ISKNV plays important roles in viral infection

Fig. 3

a The BSP results of 3 regions in the ISKNV and ISKNV-Aza genomes demonstrate the methylation level of each CpG site in these regions. b The MSP results of 6 regions in the ISKNV and ISKNV-Aza genomes. The histogram shows methylation levels in different regions of the viral genome obtained by MSP, calculated as “gray value from M primers / (gray value from M primers + gray value from U primers)”. c Immunofluorescence of MFF-1 cells infected with ISKNV (MOI = 1) at 0, 1, 2, and 4 days; blue represents nuclei, green represents DNA containing 5mC, and red represents isknvORF108L. The scale bars in the figure represent 2.5 μm. In the figure, “M Primers” indicates methylated primers, and “U Primers” indicates unmethylated primers.

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