Fig. 2: Androgen receptor antagonism increases ST3Gal1 and α2-3-linked sialoglycans (a) ST3GAL1 mRNA expression in LNCaP cells following 10 µM enzalutamide treatment measured by RT-qPCR. | Communications Biology

Fig. 2: Androgen receptor antagonism increases ST3Gal1 and α2-3-linked sialoglycans (a) ST3GAL1 mRNA expression in LNCaP cells following 10 µM enzalutamide treatment measured by RT-qPCR.

From: ST3 beta-galactoside alpha-2,3-sialyltransferase 1 (ST3Gal1) synthesis of Siglec ligands mediates anti-tumour immunity in prostate cancer

Fig. 2

N = 3 biologically independent samples. b ST3Gal1 protein expression in LNCaP cells following 10 µM enzalutamide treatment quantified using a pre-validated ELISA. N = 3 biologically independent samples. c MAL-II lectin detection of α2-3-sialylation in LNCaP cells following 10 µM enzalutamide treatment measured by flow cytometry. Representative histogram shown and bar chart of median fluorescent intensities. Histogram representative of N = 3 biologically independent samples. d Experimental design for TRAMP-C2 subcutaneous allografts in C57BL/6 mice treated with enzalutamide 20 mg/kg daily by oral gavage. Schematic created with BioRender.com e Tumour growth curves for subcutaneous allografts with 20 mg/kg enzalutamide treatment or vehicle control (N = 6 mice/group). f Tumour weights when tumours were harvested following 7 days enzalutamide treatment or vehicle control (N = 6 mice/group). g RT-qPCR analysis of St3gal1 mRNA expression in TRAMP-C2 subcutaneous tumours following 7 days vehicle or enzalutamide treatment. h MAL-II lectin flow cytometry for cell surface α2-3-sialylation following 10 µM enzalutamide treatment for TRAMP-C2 cells. Representative histogram of N = 3 biologically independent samples and bar chart with median fluorescent intensities shown. (i) MAL-II lectin immunofluorescence detection of α2-3- linked sialic acid (red) expression in FFPE subcutaneous TRAMP-C2 tumours treated with vehicle or enzalutamide. Data are corrected total cell fluorescence (CTFC). Representative images shown. For each tumour 3 images were taken and quantified. Scale bar = 100 µm. j (t-distributed stochastic neighbourhood embedding) tSNE maps of flow cytometric analysis of immune populations in subcutaneous allografts from vehicle and enzalutamide-treated mice (N = 4). k ST3Gal1 gene expression levels determined by RNA sequencing of match biopsies pre and post enzalutamide treatment (N = 5). Significance tested using two-way t-tests. Statistical significance is shown as * p < 0.05, ** p < 0.01, *** p < 0.001 and **** p < 0.0001. Error bars show standard error of the mean.

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