Fig. 2: The analysis of pairing in pachytene oocytes (a1–d3) and spermatocytes (e1–e3) of C. hankugensis (a1–a3) and triploid HHL hybrids (b1–e3).
From: A cyclical switch of gametogenic pathways in hybrids depends on the ploidy level
Synaptonemal complexes were visualized using immunostaining of lateral (SYCP3 protein, green) (a1, b1, c1, d1, and e1) and central (SYCP1 protein, red) (a2, b2, c2, d2, and e2) components. Corresponding merged figures (a3, b3, c3, d3, and e3) also include DAPI staining (blue). Accumulation of SYCP3 and SYCP1 proteins (indicated by thick arrows) allows distinguishing bivalents, while univalents accumulate only SYCP3 protein (indicated by arrowheads). Pachytene oocytes of C. hankugensis exhibit 24 fully paired bivalents (a1–a3). In triploid hybrids, we observed pachytene oocytes with 24 bivalents and 25 univalents (b1–b3), oocytes with 24 bivalents (c1–c3), and oocytes with 25 univalents (d1–d3). Triploid hybrid males exhibit pachytene oocytes only with the aberrant pairing of several bivalents and univalent (e1–e3). Scale bar = 10 µm.
