Fig. 3: The analysis of crossover loci in pachytene oocytes (a1–d3, f1–f3) and spermatocytes (e1–e3) from gonads of C. hankugensis (a1–a3), triploid HHL hybrids (b1–e3) and diploid hybrid (f1–f3).
From: A cyclical switch of gametogenic pathways in hybrids depends on the ploidy level
Crossover loci were detected by MLH1 protein (indicated by thin arrows, red) (a2, b2, c2, d2, e2, and f2) on lateral components of synaptonemal complexes (SYCP3 protein, green) (a1, b1, c1, d1, e1, and f1). Corresponding merged figures (a3, b3, c3, d3, e3, and f3) also include DAPI staining (blue). MLH1 bindings (indicated by thin arrows, red) are located on bivalents (indicated by thick arrows) and do not accumulate on univalents (indicated by arrowheads). Pachytene oocytes of C. hankugensis exhibit 24 fully with at least one crossover locus per bivalent (a1–a3). In triploid hybrids, oocytes with 24 bivalents and 25 univalents have MLH1 signals only on bivalents (b1–b3). Oocytes with exclusively 24 bivalents (c1–c3) have recombination signals on each bivalent, while oocytes with exclusively 25 univalents (d1–d3) do not have crossover locus. MLH1 immunostaining demonstrates the presence of crossover in individual bivalents formed in a triploid hybrid male (e1–e3) and pachytene oocytes with an unduplicated genome (f1–f3). Scale bar = 10 µm.
