Fig. 5: The senescence-associated inflammatory phenotype is more robust in RPTECs and HCMV replication-dependent.
From: Human cytomegalovirus infection triggers a paracrine senescence loop in renal epithelial cells
a The protein concentration of IL-6 and IL-8 was evaluated by ELISA in supernatants from HCMV- or mock-infected RPTECs, ARPE-19 and HFFs at 3dpi (MOI 1, 3 and 0.5, respectively). Data are expressed as mean values ± SD of three independent experiments (*P < 0.05, **P < 0.01, multiple unpaired t-test). b qPCR analysis of KI67 and LAMINB1 mRNA expression levels from total RNA extracts harvested at 3 dpi. Values are normalized to 18 S gene expression and plotted as fold induction over mock-treated cells. qRT-PCR data are presented as mean values from biological triplicates. Error bars indicate SD (*P < 0.05, multiple unpaired t-test). c UVB inactivated virus (UVB-HCMV) was used to evaluate the impact of HCMV replication on the release of IL-6 by ELISA (left panel) or on EdU incorporation (right panel) in RPTECs, ARPE-19 and HFFs. Data are expressed as mean values ± SD from three independent experiments.
