Fig. 7: HCMV infection triggers the expression of the phosphorylated form of histone H2AX (γH2AX) in bystander uninfected RPTECs but not HFFs or ARPE-19.
From: Human cytomegalovirus infection triggers a paracrine senescence loop in renal epithelial cells
Representative immunofluorescence co-staining for γH2AX (in green) and HCMV immediate early antigen (IEA, in red) in mock- or HCMV-infected RPTECs (a), ARPE-19 (b) and HFFs (c) at 3dpi. Cell nuclei are visualized by DAPI staining (blue). Scale bars, 30 μm. d Histograms represent the number of γH2AX-positive nuclei and IEA positive cells per field in HCMV-infected cells. Three random fields of view per cell line from 3 different experimental replicates (at ×63 magnification) were captured, and the cell counts were graphed (*P < 0.05, multiple unpaired t-test).
