Fig. 8: HCMV infection triggers the expression of p16INK4a in bystander uninfected RPTECs but not HFFs or ARPE-19.
From: Human cytomegalovirus infection triggers a paracrine senescence loop in renal epithelial cells
Representative immunofluorescence staining for p16INK4a (in red) and HCMV immediate early antigen (IEA, in green) in mock- or HCMV-infected RPTECs (a), ARPE-19 (b) or HFFs (c). Cell nuclei were visualized by DAPI (blue). Scale bars, 30 μm. d Histograms representing the number of p16INK4a and IEA positive cells per field in HCMV-infected cells. Three random fields of view per cell line from 3 different experimental replicates (at ×63 magnification) were captured, and the cell counts were graphed (*P < 0.05, multiple unpaired t-test).
