Fig. 4: Mean nuclear dry-mass density distribution.

a 3D reconstructions of normalized mean nuclear dry mass density map for selected embryos from Supplementary Fig. 6, enclosed by red boxes (sick), orange boxes (intermediate), and green boxes (healthy). b Mean nuclear dry mass density differences between TE versus ICM nuclei for healthy, intermediate, and sick class of embryos, showing significant differences for healthy (p = 3.41e−27, with 768 TE and 2007 ICM nuclei), and intermediate class (p = 2.71e−20, with 1019 TE and 2808 ICM nuclei), but nonsignificant (ns) differences for sick class (p = 0.071, with 340 TE and 846 ICM nuclei). c Mean nuclear dry mass density differences between TE versus ICM nuclei for blastocyst and cleavage-stage embryos, showing significant differences for blastocyst (p = 8.11e−39, with 1570 TE and 4538 ICM nuclei), but nonsignificant (ns) differences for cleavage stage embryos (p = 0.0105, with 557 TE and 1123 ICM nuclei), with significance threshold alpha set at p = 0.0001 for both (b) and (c). Kruskal Wallis non-parametric test was performed to determine statistical significance. A total of 152 embryos were analyzed for (b) and (c). Colorbar in a represents normalized mean nuclear dry mass density. Median value is represented by the line inside each box, whiskers extend to the maximum and minimum data point within 1.5 times the inter quartile range (1.5*IQR) from the respective quartile (box edge), with outliers represented by black dots. Raw data is overlayed as colored dots as per the legend.