Fig. 2: Swelling in response to CFTR modulator treatment in intestinal organoids with various CFTR genotypes. | Communications Biology

Fig. 2: Swelling in response to CFTR modulator treatment in intestinal organoids with various CFTR genotypes.

From: OrgaSegment: deep-learning based organoid segmentation to quantify CFTR dependent fluid secretion

Fig. 2

a Overview of the FIS assay timeline and representative images of PDIOs at various stages of the FIS assay; directly after VX-445/VX-661 addition (t = 0 h), upon VX-770 and forskolin treatment (t = 24 h) and 1 h after forskolin treatment (t = 25 h), for PDIOs with various genotypes. b. Quantification of SLA at the various stages of the FIS assay, in the presence of DMSO (control) and VX-445/VX-661/VX-770. Error bars represent standard deviation and significance, calculated using two-tailed t-tests, is indicated where significant, (*p < 0.05, **p < 0.01, ***p < 0.001).

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