Fig. 3: The DIS experimental pipeline and typical swelling distribution of individual organoid structures within an experiment.
From: OrgaSegment: deep-learning based organoid segmentation to quantify CFTR dependent fluid secretion
a Overview and timeline of a typical DIS experiment. b Step-by-step schematic overview of a DIS experiment and analysis. Starting with (1) imaging and image exporting to JPEG, followed by (2) organoid segmentation using OrgaSegment, (3) particle tracking, (4) calculation of swelling per organoid, (5) exclusion of outliers, and finally (6) calculating mean swelling per condition and per experiment (assay plate/biological replicate). c Distribution of organoid swelling for individual F508del/class I and F508del/F508del PDIO structures in the presence of DMSO or VX-445/VX-661/VX-770 treatment. Two different donors are measured per genotype in biological triplicate. d Overview of the swelling distribution of randomly selected organoids with various bin sizes, for F508del/class I and F508del/F508del PDIOs in the presence of DMSO or VX-445/VX-661/VX-770 treatment.
