Fig. 2: Point mutations in Hexads B and C affect HAP expression in the mouse heart. | Communications Biology

Fig. 2: Point mutations in Hexads B and C affect HAP expression in the mouse heart.

From: Unraveling the evolutionary origin of the complex Nuclear Receptor Element (cNRE), a cis-regulatory module required for preferential expression in the atrial chamber

Fig. 2

A Strategy for the mutation of Hexad A (Mut A). B Mouse line 14 (Mut A) at 10.5 days post coitum (dpc), showing atrial-specific expression of HAP. C Strategy for the mutation of Hexad B (Mut B). D Mouse line 17 (Mut B) at 10.5 dpc, showing expression of HAP. E–G Time course (10.5 dpc to 12.5 dpc) of cardiac expression in both chambers (atrium and ventricle) in mouse line 17 (Mut B). H Comparison of HAP assays in homogenates of atrial and non-atrial cardiac tissues from Mut B line 17 (n = 38) and SMyHC III line 5 (n = 18) in 10.5 dpc embryos, one-way ANOVA with Bonferroni post-hoc test, ****p < 0.0001. I Strategy for the mutation of Hexad C (Mut C). J Mouse line 5 (Mut C) at 9.5 dpc. K Isolated heart from the SMyHC III::HAP line at 10.5 dpc. L Isolated heart from a wild-type littermate at 10.5 dpc. M Isolated heart from mouse line 5 (Mut C) at 10.5 dpc. N Comparison of HAP assays in homogenates of atrial and non-atrial cardiac tissues from Mut C line 5 (n = 15) and SMyHC III line 5 (n = 18) in 10.5 dpc embryos, one-way ANOVA with Bonferroni post-hoc test, ****p < 0.0001. O Strategy for the mutation of the non-Hexad control (Mut S) in the spacer sequence between Hexads B and C. P Representative mouse line 14 (Mut S) at 10.5 dpc, showing atrial-specific HAP expression.

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