Fig. 2: Cells selectively uptake microdiamonds than latex beads, and actively transport particles through actin filaments.
From: Cell-particles interaction – selective uptake and transport of microdiamonds
a Cells with both microdiamonds (MDs) (red) and latex beads (LBs) (green) after 24 h of incubation, fixed and stained for nucleus and actin (both in blue), show more MDs than LBs inside the cells around the nucleus. Replications and number of cases are 4 and 70, respectively. b Full field of view of the cell at the beginning of uptake. c Time frames of the uptake of the MD by the cell which starts 1 h after adding particles to the sample show that the cell extends the protrusion to the MD to uptake it while leaving the LB outside the cell. In c (2), the cell is connected to the LB by protrusion, but it does not lead to the uptake of this particle. Replications and number of cases for (b–c) are 4 and 14, respectively. The results of selective uptake have been taken from a full field of view (300*300 µm), one example can be seen in the Supplementary Fig. 4. d Full field of view of the investigated live cell transfected with EGFP-F-tractin and MDs deposited around it. e (1–6) The time frames of the cropped area marked in (d) show that the cells transport the MDs along the actin fiber; this process started 50 min after adding the particles. Replications and number of cases for (d–e) are 3 and 7, respectively.
