Fig. 4: Myosin-X accumulates around the particles within normal and Bleb-treated cells.
From: Cell-particles interaction – selective uptake and transport of microdiamonds
a Control cell without particles and without myosin-II inhibition, stained for myosin-X (green) and actin (blue) shows a normal distribution of myosin-X protein in the cell and at the tips of filopodia. b Results of untreated cells incubated with microdiamond particles (MDs) (red). There is accumulation of myosin-X around MDs inside the cell. Replications and number of cases for (a, b) are 7 and 34, respectively. c Myosin-X accumulates around latex beads (LBs) (red) inside the untreated cell. There is a higher accumulation of myosin-X around a cluster of six particles than around two or a single particle. d An orthogonal view of (c) shows the aggregation of myosin-X around the particles in both the xz and yz directions. Replications and number of cases for (c, d) are 3 and 7, respectively. (e) and (g) Bleb-treated cells show myosin-X in the vesicles (white arrows in f and h) along the cell tails after treatment for 6 h and 24 h, respectively. i, j Accumulation of myosin-X around the particles in cells after 6 h of treatment with Bleb. Replications and number of cases for (e–j) are 3 and 34, respectively.
