Fig. 5: Myosin-X shows a colocalization with microtubules in the areas where particles are present.

a Cross-staining for actin (blue), microtubules (MTs) (red), and myosin-X (green) in cells incubated for 24 h with non-fluorescent microdiamonds (MDs). Myosin-X is mostly localized with the MT in areas where there are MDs. Separated channels of this panel can be seen in Supplementary Fig. 12. b The cropped part of (a) shows that myosin-X is localized with MTs-rich areas, and not with actin reach areas (as shown by dashed lines). Replications and number of cases for (a, b) are 4 and 33, respectively. c The control sample without particles with the same staining shows no colocalization between the MT and myosin-X in the cell in the center of the image. d Evaluation of the accumulation of myosin-X (green) around latex beads (LBs) (blue) in four selected areas with microtubules (MT) (red) shows that in three areas, there is myosin-X around visible LBs inside the cell but shows no myosin-X in the last area without particles. e In the magnification of the four areas of image d for different imaging channels, the amount of myosin-X is smaller around one particle in area 3 than in area 1, which contains five particles. Interestingly, the amount of myosin-X is significantly reduced in area 4, even with the denser MT but without beads. Separated channels of panel d can be seen in Supplementary Fig. 13 where we can see myosin-X tracks along MT. Replications and number of cases for (d, e) are 3 and 10, respectively. f and g The control sample with the same staining as image d does not show any accumulation or colocalization of myosin-X and the MT without the presence of particles.