Fig. 6: Myosin-X and EEA1 show colocalization around particles inside the cells.
From: Cell-particles interaction – selective uptake and transport of microdiamonds
a Control staining of cells with EEA1 (red) and myosin-X (green) shows no accumulation or colocalization between these two proteins. b After 6 h of incubation of cells with microdiamonds (MDs) (green), the cells fixed and stained for EEA1 and actin (blue) show endosomes around MDs. c Cells after 12 h of incubation with non-fluorescent MDs fixed and stained for EEA1 (red) and myosin-X (green) show an accumulation of both proteins around MDs. The white arrows indicate other MDs inside the cell, in which there is no separate accumulation of any of the two proteins. This result suggests a functional relationship between myosin-X and EEA1. d The magnified selected area in c shows a dense accumulation of myosin-X and EEA1 around MDs. Replications and number of cases for (b–d) are 4 and 33, respectively.
