Fig. 6: Post-ischemic ubiquitination reversibly suppresses CaMKII and PKC activities at the PSD. | Communications Biology

Fig. 6: Post-ischemic ubiquitination reversibly suppresses CaMKII and PKC activities at the PSD.

From: Post-ischemic ubiquitination at the postsynaptic density reversibly influences the activity of ischemia-relevant kinases

Fig. 6

a Cortical PSD-lysates from sham and MCAO-treated mice were assessed for CaMKII and PKC downstream target phosphorylation. Phospho-specific vs total protein bands were quantified. GluN2B: *P = 0.0162 from s; nNOS: *P = 0.0046 from s, #P = 0.0426 from s; one-way ANOVA with Bonferroni test; n = 5 animals/group. b Cortical PSD-lysates from sham and MCAO-treated mice were assessed for CaMKII activity (*P < 0.0001, two-tailed unpaired t-test; n = 9 animals/group). c Same as in b, but for PKC (*P < 0.0001, two-tailed unpaired t-test; n = 8 animals/group). d Post-ischemic PSD lysates were treated with recombinant deubiquitinase USP2, and CaMKII activity was reassessed and quantified (*P = 0.0026, two-tailed paired t-test; n = 6 animals). e Same as in d, but for PKC (*P = 0.0012, two-tailed paired t-test; n = 8 animals). c contralateral, i ipsilateral, S serine, s sham. Data are expressed as mean ± s.e.m.

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