Fig. 7: Post-ischemic ubiquitination reduces PSD-associated Cdk5 activity. | Communications Biology

Fig. 7: Post-ischemic ubiquitination reduces PSD-associated Cdk5 activity.

From: Post-ischemic ubiquitination at the postsynaptic density reversibly influences the activity of ischemia-relevant kinases

Fig. 7

a Phosphorylation levels of Cdk5 downstream targets were determined in PSD lysates of sham and MCAO-treated animals. Tau: *P = 0.0137 from s, #P = 0.0055 from s; Crmp2: *P = 0.0496 from s and P = 0.0430 from c; one-way ANOVA with Bonferroni test; n = 4–5 animals/group. b Cortical PSD-lysates from sham and MCAO-treated mice were assessed for Cdk5 activity (*P < 0.0001, two-tailed unpaired t-test; n = 6 mice/group). c Cdk5 activity was measured in post-ischemic PSD lysates untreated and treated with recombinant deubiquitinase USP2 (*P = 0.0057, two-tailed paired t-test; n = 6 animals). c contralateral, i ipsilateral, S serine, s sham. Data are expressed as mean ± s.e.m.

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