Fig. 8: Ubiquitination reversibly activates PSD-located Pyk2 after ischemia. | Communications Biology

Fig. 8: Ubiquitination reversibly activates PSD-located Pyk2 after ischemia.

From: Post-ischemic ubiquitination at the postsynaptic density reversibly influences the activity of ischemia-relevant kinases

Fig. 8

a Phosphorylation of Pyk2 kinase target proteins was analyzed in PSD extracts. GluN2B: *P = 0.0016 from s and *P = 0.0001 from c; Src: *P < 0.0001 from s and *P = 0.0003 from c; Tau: *P < 0.0001 from s and c; one-way ANOVA with Bonferroni test; n = 5 animals/group. b Pyk2 tyrosine phosphorylation was assessed on Y402 (*P = 0.0184 from s; **P = 0.0040 from s and **P < 0.0001 from 30 min rep; one-way ANOVA with Bonferroni test; n = 4–6 animals/group). c Overall Pyk2 tyrosine phosphorylation was assessed after Pyk2 pull down with a phospho-tyrosine-specific antibody (*P = 0.0089 from s; **P = 0.0005 from s; one-way ANOVA with Bonferroni test; n = 4-6 animals/group). d Phosphorylation of immobilized recombinant his-GST-Src by Pyk2 derived from sham and MCAO lysates untreated or treated with recombinant USP2 was determined. *P = 0.0152 from s; #P = 0.0242 from i/untreated; one-way ANOVA with Bonferroni test; n = 4 animals/group. c contralateral, i ipsilateral, S serine, s sham, Y tyrosine. Data are expressed as mean ± s.e.m.

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