Fig. 2: Post-ischemic injury disrupts cerebral circulation independent on the anisotropic arteriolar diameter changes. | Communications Biology

Fig. 2: Post-ischemic injury disrupts cerebral circulation independent on the anisotropic arteriolar diameter changes.

From: Ca2+ oscillation in vascular smooth muscle cells control myogenic spontaneous vasomotion and counteract post-ischemic no-reflow

Fig. 2

a Scheme of timepoints for MCAO surgery, 2PLSM and experimental design. b Representative frame-scan images of DiO-labeled blood cell trajectories in MCA before and after (Occ.2hRep.22 h) ischemic stroke. The magenta solid line indicates the line-scan area in (c). c Representative line-scan images of DiO-labeled blood cell trajectories in MCA before and after (Occ.2hRep.22 h) ischemic stroke. The white arrow indicates the flow direction, which was reversed during occlusion and recovered after reperfusion compared with before ischemia. d Statistical analysis of the arteriolar blood flow velocity (mm/s) in MCA before and after ischemic (Occ.2hRep.22 h) stroke (N = 4, n = 48 vessels). The blood flow velocity was analyzed by calculating the slope of the DiO-labeled blood cell trajectory in the kymographs in (c). e Representative line-scan images of RhoB-labeled capillary blood flow before and after (Occ.2hRep.22 h) ischemic stroke. The shaded streaks indicate the RBC motion trajectory, and the red streaks indicate the tracer-filled capillary lumen. The white arrow indicates the flow direction. f, g Capillary blood velocity and blood flow changes curve during ischemic stroke, data were analyzed by calculating the streaks slope and the cell number in the kymographs (N = 7 mice, n = 39 vessels). h Representative images of MCA changes before and after (Occ.2hRep.22 h) ischemic stroke. Examples of constricted, unchanged, and dilated arterioles are displayed. i, j Statistical analysis of paired arteriolar diameter (h) and diameter change rate (i) before and after (Occ.2hRep.22 h) ischemic stroke (N = 6 mice, n = 90 vessels). k Change rate analyses for arteriolar diameter, arteriolar blood velocity, capillary blood velocity, and CBF (including LSCI and LDF) changes before and after (Occ.2hRep.22 h) ischemic stroke. All data were normalized to the basal value before ischemic stroke. Data are expressed as the mean ± SEM. Statistic for (h), paired t tests were used. Otherwise, data were analyzed using one-way ANOVA, followed by Tukey’s post hoc analysis.

Back to article page