Fig. 1: Activation of an EC transcription program during cardiogenic mesoderm differentiation of mESC.
From: Endothelial gene regulatory elements associated with cardiopharyngeal lineage differentiation
a Schematic illustration of the EC differentiation protocol from mESCs. b Expression of pluripotency (Oct3/4; Nanog; Rex1), mesodermal (Brachyury; Mesp1; Pdgfrα; Gata4), endothelial marker genes (Pecam1; Eng; Kdr; Cdh5; Nos3; Flt1; Gata6; Notch1), and the CPM marker Tbx1 during differentiation by RT-PCR. Gapdh was used as a normalizer. The molecular weight marker is the 100 bp ladder. Uncropped photographs of gels are reported in Supplementary Fig. 1. c RNA-seq volcano plot of differentially expressed genes (DEGs) in d4 vs d2 samples in two biological replicates. Genes downregulated at d4 (n. 731) are indicated in blue and genes upregulated are in red (n. 1088). We indicate examples of endothelial marker genes (Flt1; Kdr; Ets1; Fli1; Gata4; Pecam1; Cdh5; Etv2; Gata6; Gata1; Erg; Tie1; Gata2, Notch1).
