Fig. 7: The deletion of the Notch1 EC-RE affected the development of a vascular-like network in differentiating mESC.
From: Endothelial gene regulatory elements associated with cardiopharyngeal lineage differentiation
a Immunofluorescence images showing PECAM1 expression (in green) on cardiac gastruloids at 168 h, using WT and mESC Notch1-∆enh.in15 (clones #7G; #11B). Images were obtained with Nikon A1 Confocal Microscopy. Scale bars: 100 µm. b In vitro tube formation assay (Matrigel) of d8 WT and mESC Notch1-∆enh.in15 (clones #7G; #11B) plated for 24 h. Scale bars, 100 µm. Quantification of branch points from five independent experiments. p value (**) <0.01 obtained using the parametric paired t-test, one-tailed. Error bars: SD.
