Fig. 1: VPS37A1–148 selectively interacts with highly curved membranes.
a Gel images of liposome flotation assays for VPS37A1–148 mixed with sonicated or extruded liposomes with membrane pore sizes of 50, 100, or 200 nm (POPC:DOPG:DOPE = 3:2:5, protein:lipid = 1:400, molar ratio). T, M, and B represent the top, middle, and bottom layers after centrifugation. The protein marker is indicated by m. The arrow indicates the lipid band. The amount of VPS37A1–148 in the top layer relative to that in the bottom layer (T/B) is quantitated by ImageJ and plotted in b. b Plots of VPS37A1–148 in top layer versus bottom layer (T/B) for extruded liposomes (POPC:DOPG:DOPE = 3:2:5) with membrane pore sizes of 50, 100, or 200 nm, and sonicated liposomes containing 0, 10, 30, or 50% PE (molar ratio, referred to as S-0%, S-10%, S-30%, and S-50%, respectively). Data are presented as mean ± SD (standard deviation). Quantifications were obtained from three separate measurements (n = 3). c Gel images of liposome flotation assays for VPS37A1–148 mixed with sonicated liposomes consisting of 20% DOPG and 0, 10, 30, or 50% of PE (molar ratio). Their plots are shown in b. The protein marker is indicated by m. The arrow indicates the lipid band. d Gel images of liposome flotation assays for VPS37A1–148 mixed with sonicated liposomes containing 0 or 55% of DOPE (molar ratio) without negatively charged lipids, and with sonicated liposomes (POPC:DOPS:DOPE = 3:2:5, protein:lipid = 1:400, molar ratio). Samples with VPS37A1–148 proteins alone (-liposomes) or liposomes alone (-protein) were performed as controls. The protein marker is indicated by m. The arrow indicates the lipid band.
