Fig. 1: Organ specificity of donor ECs.
From: Brain-derived endothelial cells are neuroprotective in a chronic cerebral hypoperfusion mouse model
a Outline of the transplantation experiment (a–f). b Representative images of the progress of transplanted ECs derived from C57BL/6-Tg (CAG-EGFP) mice in the brain. The endogenous fluorescence intensity of GFP was observed under TPEM. Scale bar, 500 μm. c Bar graph showing the mean of the regenerated vascular area by GFP-positive ECs (on days 7 and 21) and the growth rate (from days 7 to 21) of GFP-positive ECs (n = 3 per group). The growth rate was defined as the percentage increase in vascular area from days 7 to 21. Data are presented as mean ± SEM. d Representative images stained with anti-GFAP pAb (Alexa Flour 546) on day 28 after transplantation of ECs. Scale bar, 50 μm. e Bar graph showing the mean volume and total amount of fluorescence intensity of GFAP-positive cells (n = 3 per group) suggesting the degree of fibrosis. Data are presented as mean ± SEM. f Bar graph showing the mean novelty score on days 14 and 28 (n = 3 per group). Data are presented as mean ± SEM. *, P < 0.05; **, P < 0.01; ns not significant. BCAS bilateral common carotid artery stenosis, CAG-EGFP CAG-enhanced green fluorescent protein, EC endothelial cell, GFAP glial fibrillary acidic protein, GFP green fluorescent protein, NEG negative control mice received no cell transplants, NOR novel object recognition, ns not significant, pAb polyclonal antibody, TPEM two-photon excitation microscopy.
