Fig. 6: Rescue of fibrosis by CD157-positive ECs.
From: Brain-derived endothelial cells are neuroprotective in a chronic cerebral hypoperfusion mouse model
a Quantification of vascular colony forming–areas by GFP-positive ECs. 5 × 103 CD157+ or CD157- ECs from brains were seeded on OP9 feeder cells for 10 days and stained with anti-CD31 antibody (n = 3 per group). b, c Vascular regeneration by transplanted CD157-positive or -negative ECs from GFP mice into a chronic cerebral hypoperfusion model. Representative images of vascular regeneration observed under TPEM in the same region using the same mice. b Vascular regeneration was evaluated on days 7 or 21 after transplantation. The bar graph shows the mean area (days 7, 21) of GFP-positive cells (n = 4 per group) (c). Data are presented as mean ± SEM. d, e Rescue of brain fibrosis by CD157-positive ECs but not by CD157-negative ECs in a chronic cerebral hypoperfusion model. Representative images stained with anti-GFAP pAb (Alexa Flour 546). Scale bar, 50 μm. e Bar graphs showing the mean volume and total amount of fluorescence intensity of GFAP-positive cells (n = 4 per group). Data are presented as mean ± SEM. f Bar graphs showing the mean novelty score on days 14 and 28 (n = 4 per group) after the transplantation of ECs. Data are presented as mean ± SEM. **, P < 0.01; ***, P < 0.001; ****, P < 0.0001; ns not significant. EC endothelial cell, GFAP glial fibrillary acidic protein, GFP green fluorescent protein, pAb polyclonal antibody, TPEM two-photon excitation microscopy.
