Fig. 3: Iodido agents induce cellular senescence in GI cancer cells.
a Mean fold change ± SD in CellEvent Senescense Green Probe detection, determined by flow cytometry, in AGS, MKN45 and PANC1 cells treated with CDDP, I5 or I6 IC50 doses for 24 h. *p < 0.05, **p < 0.01, ***p < 0.001, as determined by unpaired two-sided Student’s t-test, compared to untreated (C) set as 1.0. b AGS, MKN45 and PANC1 cells were treated with CDDP, I5 or I6 (IC50 concentration, see Methods) for 3 h. γ-H2AX foci (green fluorescence) were detected by immunofluorescence using DAPI to stain nuclear DNA (blue fluorescence). Representative images of each condition were taken. Graphs represent the percentage of nuclei with <15, between 16 and 30, between 31 and 45, between 46 and 60 and >60 γ-H2AX foci per nuclei for each condition. Data represent the mean values obtained in three experiments performed in duplicate. c RNA was isolated from AGS, MKN45 and PANC1 cell lines stimulated with a 24 h treatment of the complexes. CDKN1A, IL-6 and MMP1 were quantified by RT-qPCR. Target gene expression was normalized to GAPDH. All the experiments were performed three times with IC50 concentrations of each compound used in all the assays. The statistical significance was evaluated with Student’s 2-tailed t-test (*p < 0.05, **p < 0.01, ***p < 0.001) compared to untreated (C) set as 1.0.
