Fig. 5: Functional consequences of elevated GC: precocious feeding followed by physical decline.
From: Elevated glucocorticoid alters the developmental dynamics of hypothalamic neurogenesis in zebrafish
a Analysis of feeding behaviour reveals that 5 dpf star:bPAC+ larvae eat more (N = 12, t = −2.1694, df = 21.996, p = 0.04113). b Analysis of feeding behaviour in 13 dpf wild-type and star:bPAC+ larvae reveals that food intake is significantly reduced (N = 14, t = 2.5626, df = 24.299, p = 0.017). c In 13 dpf wild-type larvae, food intake correlates positively with body size (linear regression, R-squared: 0.3619, F: 6.805 on 1 and 12 DF, p value: 0.02286), however in star:bPAC + , there is no significant correlation (R-squared: 0.1877, F: 2.773 on 1 and 12 DF, p value: 0.1218). d star:bPAC+ larvae are significantly smaller than wild types at 28 dpf and 120 dpf, but not at 5 dpf or 13 dpf (Two-way ANOVA with Tukey’s test, ***p < 0.001 for 28 and 120 dpf, 5 dpf: N = 12, 13; 13 dpf N = 9, 10; 28 dpf N = 13, 15; 120 dpf N = 15,15 for wild type and bPAC+ respectively). e Percentage of embryos that were fertilised by adult male star:bPAC+ fish is significantly lower compared to wild types (N = 19 wild type, N = 16 bPAC+ pairs, t = 2.7181, df = 31.072, p = 0.01064). f Percentage of star:bPAC+ fish that survived to 2 months is significantly reduced, compared with wild types (Wilcoxon test, W = 65.5, p = 0.004315, N = 9 wild type N = 8 bPAC+ stocks).
