Fig. 2: GR interacts and recruits Kapβ2 in neurons.
a Confocal images of rat cortical neurons in culture transduced with GFP and GR50 and stained for Kapβ2 and Map2. Imaris colocalization channel shows the amount of GFP colocalized with Kapβ2. Scale bar = 10 μm. b Pearson’s coefficient measured in each neuron transduced with the lentivirus GR50 construct (Flag-GR50-GFP). Data are represented as mean ± S.E.M. (n = 3 biological replicates, m > 5 neurons, Student t-test, *P < 0.05). c Confocal images of the cortex and spinal cord tissues of GR50 mice stained for Kapβ2. Green is GFP reporting for GR50, red is Kapβ2, and magenta is NeuN. Imaris colocalization shows the amount of GFP colocalized with Kapβ2. Scale bar = 30 μm. d Pearson’s coefficient measured in the cortex and spinal cord neurons of GR50 mice. Data are represented as mean ± S.E.M. (n = 3 biological replicates, m > 15 neurons). e Western blot analysis of the immunoprecipitation assay was performed in rat cortical neurons transduced with GR50 and treated with arsenite (0.5 mM; 30 min). Blots were probed for GFP and Kapβ2. Total protein staining was used as the loading control. SE short exposure time, LE long exposure time. f Western blot of the immunoprecipitation assay performed in the cortex or spinal cord tissue of nTg or GR50 mice. Blots were probed for GFP and Kapβ2. Total protein staining was used as the loading control. SE short exposure time, LE long exposure time.
