Fig. 3: Silencing of Kapβ2 increases death risk in the presence of GR.
a Western blot showing Kapβ2 expression in neurons treated with Smart pool siRNA (100 μM). Blots were probed for Kapβ2. Total protein staining was used as the loading control. b Kapβ2 levels in primary neurons treated with Smart pool siRNA 100 μM or scramble. Data are represented as mean ± S.E.M. (n = 3 biological replicates, Mann-Whitney non parametric test, P < 0.05). c The plot depicts the probability of neuronal death in each group via a cumulative risk of death plot. Time-lapse experiments on rat primary cortical neurons transfected with siRNA Kapβ2 100 μM or scramble and GFP, GR50-GFP, or GR100-GFP. (n = 3 biological replicates, m > 50 neurons, Log-rank Mantel-Cox test *p < 0.05, ***p < 0.001). d Quantification of the mean fluorescence of GFP expressing neurons 48 h after transfection in cells previously treated with Kapβ2 siRNA or scramble. Data are represented as mean ± S.E.M. (n = 3 biological replicates, m > 10 neurons, One-Way ANOVA, p = n.s. not significant). e Quantification of the mean nuclear fluorescence of GFP expressing cells 48 h after transfection in cells previously treated with Kapβ2 siRNA or scramble. Data are represented as mean ± S.E.M. (n = 3 biological replicates, m > 10 neurons, One-Way ANOVA, p = n.s. not significant).
