Fig. 1: Evaluation of enzymatic activity and protein production by transformants expressing BglS in inducing and repressing conditions. | Communications Biology

Fig. 1: Evaluation of enzymatic activity and protein production by transformants expressing BglS in inducing and repressing conditions.

From: The protein methyltransferase TrSAM inhibits cellulase gene expression by interacting with the negative regulator ACE1 in Trichoderma reesei

Fig. 1

a Evaluation of β-glucosidase (pNPGase) activities under inducing condition. b Evaluation of filter paper activities (FPAs) under inducing condition. c Evaluation of extracellular protein concentrations under inducing condition. d SDS-PAGE analysis of protein production under inducing condition. e Evaluation of pNPGase activities under repressing condition. f Evaluation of FPAs under repressing condition. g Evaluation of extracellular protein concentrations under repressing condition. h SDS-PAGE analysis of protein production under repressing condition. i Western blot analysis of BglS production, the black arrows indicate BglS. The sample volume of atbg-D3 or atbg-U10 was one-tenth that of atbg-A1. j The BglS intensity ratio to DNA content. Inducing condition: 3% Avicel and 2% wheat bran were used as carbon source. Repressing condition: inducing culture added with 2% Glucose. All of the samples were collected after 5-day fermentation. All the samples were tested by three replicates. Data are represented as mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001. The molecular weight of BglS is 78.49 kDa.

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