Fig. 4: Evaluation of cellulase production of Rut-C30 and its mutants. | Communications Biology

Fig. 4: Evaluation of cellulase production of Rut-C30 and its mutants.

From: The protein methyltransferase TrSAM inhibits cellulase gene expression by interacting with the negative regulator ACE1 in Trichoderma reesei

Fig. 4

a FPAs of Rut-C30 and its transformants determined after differential deletion of TrSAM and ACE1 in 1% lactose inducing medium. b Relative expression levels of cbh1 in Rut-C30 and its transformants upon induction by 1% lactose. Rut-C30: parent strain. ∆trsam: trsam deletion strain. RK: strain harboring ACE1R383K. RQ: strain harboring ACE1R383Q. RQ-∆trsam: strain harboring ACE1R383Q and deletion of trsam. ∆ace1: ace1 deletion strain. ∆trsam∆ace1: trsam and ace1 double deletion strain. All the samples were tested by three replicates. Data are represented as mean ± SD. **P < 0.01, ***P < 0.001.

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