Table 1 Effect on FPA after deletion of genes in the lost fragment

From: The protein methyltransferase TrSAM inhibits cellulase gene expression by interacting with the negative regulator ACE1 in Trichoderma reesei

Gene No.

Annotations of the lost genes

atbg-D3

atbg-U10

Effect on FPA

123989

GH7 Cellobiohydrolase CBH1/CEL7a

-a

-

Decreased

69483

unknown protein

-

-

NSc

-

Palmitoyltransferases domain-containing protein

-

-

NS

123992

Swollenin

-

-

NS

111849

Xylanase 4

+b

-

NS

82049

unknown protein

+

-

NS

23353

Ferric reductase

+

-

NS

52315

Copper transporter

+

-

NS

69663

Esterase/lipase/thioesterase

+

-

NS

39535

mannitol 1-phosphate dehydrogenase

+

-

NS

69611

MFS transporter

+

-

NS

69500

Integral membrane protein

+

-

NS

69648

p450

+

-

NS

trsam

methyltransferase

+

-

Increased

69679

unknown protein

+

-

NS

69613

Protein of unknown function DUF227

+

-

NS

111861

hypothetical GPCR

+

-

NS

 

Ura5 (selection marker)

+

Mutated

NS

 

hph (selection marker)

+

-

NS

  1. aThe minus sign means the gene is lost in the corresponding strain.
  2. bThe plus sign means the gene is present in the corresponding strain.
  3. cNS means there is no significant difference between parent and deletion strain.