Fig. 5: Activity of promoter PSCBV-YZ2060 and eight mutants of this promoter in Arabidopsis protoplasts.
a Schematic map of promoter PSCBV-YZ2060 and eight of its mutants. T, *, and red dotted lines represent the transcription start site, the TATA box, and the deletion sites, respectively; + and – signs indicate presence and absence of cis-acting regulatory elements, respectively. Nucleotides written in lowercase letters represent the mutated nucleotides in the ABRE sequences. b Transcript expression of GUS gene driven by promoter PSCBV-YZ2060 and four of its mutants. Blank control = no plasmid. c Transcript expression of GUS gene driven by promoter PSCBV-YZ2060 and eight of its mutants subjected to 10 μM ABA treatment. Vector control = Ubi1:GUS. Values are the means (±standard errors) of four biological replicates and three technical replicates for each experiment. In b, mean values with the same letter are not significantly different at P = 0.05 according to Duncan’s multiple range test. In c, and for each promoter, values that are significantly different at P = 0.01 (Student’s T test) are indicated by two asterisks.
